Henry Trinder (Gloucester Rugby) and Marland Yarde (Sale Sharks) have been called up into England’s training squad for this week’s camp in Brighton.
Ben Loader (London Irish) picked up an ankle injury on Friday and will remain with the England U20 squad for further treatment.
Cameron Redpath (Sale Sharks) will report to camp on Monday for treatment on a knee injury and is not expected to train this week.
England face the Barbarians on Sunday 27 May at Twickenham Stadium (KO 1500 BST).
Following the conclusion of the Premiership semi finals next weekend, a final training squad will be confirmed to prepare for the Barbarians match.
England will play South Africa in three Tests in Johannesburg (9 June), Bloemfontein (16 June) and Cape Town (23 June). All matches kick off at 1705 local time (1605 BST).
Tickets for the Quilter Cup start at £25 for adults, £10 for children and are available at www.englandrugby.com/tickets